Paul J. Linser, Ph.D.

Professor
Anatomy and Cell Biology
Whitney Marine Laboratory 
email: pjl@whitney.ufl.edu
phone: (904) 461-4000
fax: (904) 461-4052


Research Interests - Research Projects - References - Biosketch - Links

Research Interests

I was trained as a Developmental Biologist and my broad interests focus on regulation of genes that serve to distinguish and define specific cell types in maturing tissues. My coworkers and I have defined a number of specific gene products that define cells of the vertebrate neural retina. One such gene product is the enzyme Carbonic Anhydrase which is expressed specifically by the glial cells of the retina. Other gene markers for the glial cells have been utilized in analyses of the regulation of cell phenotype maturation. My expertise with Carbonic Anhydrase has also drawn me into a project which focuses on the biology of disease vector mosquitoes and in particular larval molecular physiology. Our recent investigations have shown that mosquitoes possess as many as 14 Carbonic Anhydrase genes which are differentially expressed. Currently we are building a comprehensive physiological model of larval mosquito gut function in relation to its relatively unique alkaline digestive strategy. Mosquitoes are the number one threat to human health according to the World Health Organization with Malaria alone killing 3,000 children per day. Hence our investigations of larval mosquito biology have the potential to generate new control strategies and to help alleviate the burden of diseases vectored by mosquitoes.

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Research Projects

  • Our lab is pursuing two very different projects. The first is aimed at delineating molecular mechanisms that regulate gene cell development in the vertebrate neural retina. The second project focuses on the molecular physiology of the alkaline gut of the mosquito larva.

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References

  1. Linser, P.J., Peterson, R.E., McClilntock, J., Possley, J. and Orozco, A. The regulation of ller cell differentiation: focus on the definitive ller cell markers glutamine synthetase and carbonic anhydrase-II. J. Brain Res. 37:213-214, 1996.
  2. Linser, P.J. Cell-cell interactions as a target for teratogenic events. In: Drug Toxicity in Embryonic Development. R. Kavlock and G. Daston (eds.) Handb. Exp. Phar. 124:277-300, 1996.
  3. Fadool, J.M. and Linser, P.J. Evidence for the formation of multimeric forms of the 5A11/HT7 antigen. Biochem. Biophys. Res. Com. 229:280-286, 1996.  Pubmed
  4. Peterson, R.E., Tu, C. and Linser, P.J. Isolation and characterization of a carbonic anhydrase homologue from the zebrafish (Danio rerio). J. Mol. Evol. 44:432-439, 1997.  Pubmed
  5. Linser, P.J., Schlosshauer, B., Galileo, D.S., Buzzi, W.R. and Lewis. R. Late proliferation of Müller cell progenitors facilitates preferential targetting with retroviral vectors in vitro. Develop. Genetics. 20:186-196, 1997.  Pubmed
  6. Linser, P.J. Thyroid hormone influences carbonic anhydrase-II expression in embryonic retina. Bol. Med. Biol. 44:57-58, 1997.
  7. Linser, P.J. Trapido-Rosenthal, H.G. and Orona E. Glutamine synthetase is a glial-specific marker in the olfactory regions of the lobster (Panulirus argus) nervous system. Glia. 20:275-283, 1997.  Pubmed
  8. Orozco, A., Linser, P.J. and Valverde-R, C. Salinity modifies hepatic outer-ring deiodination (ORD) activity in Fundulus heteroclitus. Ann. N.Y. Acad. Sci. 839:409-411, 1998  Journal
  9. Linser, P.J., Carr, W.E.S., Cate, H.S., Derby, C.D. and J.C. Netherton III. Functional significance of the co-localization of taste buds and teeth in the phayngeal jaws of the largemouth bass, Micropterus salmoides. Biol. Bull. 195:273-281, 1998.  Pubmed
  10. Skeith, A., Dunlap, L., Galileo, D.S. and Linser, P.J. Inhibition of β1-integrin expression reduces clone size during early retinogenesis. Develop. Brain Res. 116:123-126, 1999.  Pubmed
  11. Zhuang, Z., Linser, P.J. and Harvey, W.R. Antibody to H+V-ATPase subunit E co-localizes with portasomes in alkaline midgut of a freshwater mosquito (Aedes aegypti). J. Exptl. Biol. 202:2449-2460, 1999.  Pubmed
  12. Orozco, A., Linser, P.J., and Valverde-R, C. Kinetic characterization of outer-ring deiodinase activity (ORD) in the liver, gill and retina of the killifish Fundulus heteroclitus. Comp. Biochem. Physiol. (B). 126:283-290, 2000.  Pubmed
  13. Peterson, R.E. Fadool, J.M., McClintock, J., and Linser, P.J. Müller cell differentiation in the zebrafish neural retina: Evidence of distinct multiple stages in cell maturation. J. Comp. Neurol. 429:530-540, 2001.  Pubmed
  14. Ochrietor, J.D., Moroz, L.L., Kadomatsu, K., Muramatsu, T., and Linser, P.J. Retinal degeneration folliwing failed photoreceptor maturation in 5A11/basigin null mice. Exptl. Eye Res. 72: 467-477, 2001 .  Pubmed
  15. Boudko, D.Y., Moroz, L.L., Linser, P.J., Trimarchi, J.R., Smith, P.J.S, and Harvey, W.R. In situ analysis of pH gradients in mosquito larvae using non-invasive, self-referencing, pH sensitive micorelectrodes. J. Exptl. Biol. 204: 691-699, 2001.  Pubmed
  16. Boudko, D.Y., Harvey, W.R., and Linser, P.J. Alkalinization by chloride/bicarbonate pathway in larval mosquito midgut. Proc. Natl. Acad. Sci. USA 98: 15354-15359, 2001.  Pubmed
  17. Corena, M.P., Seron, T.J., Lehman, H.K., Ochrietor, J.D., Kohn, A., Tu, C., and Linser, P.J. Carbonic anhydrase in the midgut of larval Aedes aegypti: Cloning, localization and inhibition. J. Exptl. Biol. 205: 591-602, 2002.  Pubmed
  18. Orozco, A., Jeziorski, M.C., Linser, P.J., Greenberg, R.M., Valverde-R, C. Cloning of the gene and complete cDNA encoding a type 2 deiodinase from Fundulus heteroclitus. General and Comparative Endocrinology 128: 162-167, 2002.  Pubmed
  19. Philp, N.J., Ochrietor, J.D., Rudoy, C., Muramatsu, T., and Linser, P.J. Loss of MCT1, MCT3 and MCT4 expression in the retinal pigmnet epithelium and neural retina of the 5A11/Basigin null mouse. Invest. Opthalmol. Vis. Sci. 44 :1305-1311, 2003  Pubmed
  20. Ochietor, J.D., Moroz, T.P., Clamp, M.F., Timmers, A.M., Muramatsu, T., and Linser, P.J.Inactivation of the Basigin gene impairs normal retinal development and maturation. Vision Research 42:447-453, 2002  Pubmed
  21. Ochrietor, J.D., Moroz, T.P., van Ekeris, L., Clamp, M.F.,Jefferson, S.C., deCarvalho, A.C.V.,Fadool, J.M., Wistow, G., Muramatsu, T., and Linser, P.J. Retina-specific eexpression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamily. Invest. Opthal. vis. Sci. 44:4086-4092, 2003  Pubmed
  22. Corena, M.P., Fiedler, M.M., van Ekeris, L., and Linser, P.J. A comparative study of carbonic anhydrase in the midgut of mosquito larvae. Comp. Biochem. Physiol.137:207-225, 2004  Pubmed
  23. Clamp, M.F., Ochrietor, J.D., Moroz, T.P., and Linser, P.J. Developmental analyses of 5A11/Basigin, 5A11/Basigin-2 and their putative binding partner MCTI in the mouse eye. Exp. Eye. Res.78:777-789, 2004  Pubmed
  24. Ochrietor, J.D., and Lincer, P.J. 5A11/Basigin gene products are necessary for proper maturation and function of the retina. Develop. Neurosci.26:380-387, 2004  Pubmed
  25. Seron, T.J., Hill, J., and Linser, P.J. A GPI-linked carbonic anhydrase expressed in the larval mosquito midgut. J. Exptl. Biol.207:4559-4572, 2004  Pubmed
  26. Linser, P.J. The molecular genetics of larval mosquito biology. Biological Control of Mosquitoes AMCA Bulletin #7 (in press)
  27. Corena, M.D.P., van Ekeris, L. Salazar, M.I., Bowers, D., Fiedler, M.M., Silverman, D.,Tu, C., and Linser, P.J. Carbonic anhydrase in the adult mosquito midgut. J. Exptl.Biol. 208: 3263-3273, 2005  Pubmed
  28. Ochrietor, J.D., Clamp, M.F., Moroz, T.P., Grubb, J.H., Shah, G.N., Waheed, A,. Sly, W.S., and Linser, P.J. Carbonic anhydrase-XIV identified as the membrance CA in mouse retina: Strong expression in Muller cells and RPE. Exp. Eye Res. 81: 492-500, 2005  Pubmed
  29. Corena, M.D.P., Linser, P.J. Larval Control Using Anti-Digestive Enzyme Compounds. Technical Bulletin of the Florida Mosquito Control Association (in press)

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Biosketch
    Education
    1974B.S., University of Cincinnati, (Biology)
    1977Ph.D., University of Cincinnati, (Developmental Biology)
     
    Academic Appointments
    1973Undergraduate Research Fellow, University of Cincinnati
    1974-1976Graduate Teaching Assistant, University of Cincinnati
    1974Research Associate, Cincinnati Children's Hospital Clinical Research Center
    1977-1980Postdoctoral Fellow (NIH), University of Chicago
    1980-1982Research Associate (Assistant Professor), University of Chicago, Department of Biology
    1982-1987Assistant Professor of Anatomy, University of Florida
    1982-1987Assistant Professor, Whitney Laboratory, University of Florida
    1987Organizer of Symposium, Annual Congress of the American Society of Zoologists
    1987Organizer of the Meeting of the South Eastern Regional Society for Developmental Biology
    1987-2000Associate Professor of Anatomy and Cell Biology, University of Florida, Whitney Lab
    1989-1990National Eye Institute (NIH) Visiting Professor
    1992-presentAffiliate Professor of Zoology, University of Florida
    1994-presentAffiliate Professor of Neuroscience, University of Florida
    1994-1997Coordinator, Whitney Laboratory Undergraduate Intern Program
    2000-presentProfessor of Anatomy and Cell Biology, University of Florida, Whitney Lab
    2001-presentAffiliate Professor of Fisheries and Aquatic Sciences, University of Florida

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Links

  1. Whitney Marine Laboratory
    The Whitney Laboratory, founded in 1974, is a research institute of the University of Florida. The mission of the Laboratory is to use marine organisms in basic biological research and to apply the results to problems of human health, natural resources and the environment. The Whitney Lab trains future experimental biologists, contributes to public education and helps formulate policy in basic research and marine science.
    http://www.whitney.ufl.edu

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Updated: Monday, January 12, 2009 at 10:58:16 AM, © 2005 University of Florida